DNA: The Master Molecule Video. (From: AN INTRODUCTION TO GENETIC ANALYSIS 6/E BY Griffiths, Miller, Suzuki, Leontin, Gelbart 1996 by W. H. Freeman and Company. • E. colichromosome is circular, double-stranded DNA (4.6x103 kilobase pairs, >1000 bp/sec) • Replication begins at a unique site (origin) • Proceeds bidirectionally until the two replication complexes meet (termination site) • DNA replication starts at many points in eukaryotic chromosomes. Vocabulary for ppt 2 Transcription and Translation • Genes Chapter 8.4 and 8.5 • DNA • RNA • Protein • mRNA • tRNA • rRNA • Transcription • RNA Polymerase • RNA bases • Exon • Intron • Amino Acid • Ribosome • Translation • Codon • Anticodon • Genetic Code Chart • Start codon • Stop Codons Microbial evolution 101. Watson and Crick proposed that one strand serves as a pattern on which another strand is built and this was later proved to be true. Process of DNA Replication Uncoil & unzip DNA molecule Enzyme (-ase) breaks weak Hydrogen Bond between bases B. DNA Replication •What are the key events of the template model for DNA replication? Priming DNA synthesis – Primase synthesizes RNA primers. Generation 1 E. Steps in DNA replication 1. DNA replication DNA structure suggests an obvious replication mechanism Watson and Crick noted that “specific [base]pairing…immediately suggests a possible copying mechanism for the genetic material” the model suggested that each strand could serve as a template for making a … A. One side of DNA molecule is a template for making the other side (strand) B. DNA Replication is “Semi-conservative” • Each 2-stranded daughter molecule is only half new • One original strand was used as a template to make the new strand 7. Process of DNA Replication 2. Prokaryotic cell. Fundamentals: Nucleic acids, DNA replication, transcription, translation and application to molecular detection. One side of DNA molecule is a . DNA and Replication Notes pdf: File Size: 236 kb: File Type: pdf: Download File. Enzyme brings in complementary N-bases B. Replication copies the genetic information. ppt: Download File. DNA Replication • The copying of DNA is remarkable in its speed and accuracy • Involves unwinding the double helix and synthesizing two new strands. 2. DNA Replication Complementary base pairs of the nucelotides in DNA are important because it serves as a basis for making an exact copy of the DNA when a cell is divided. DNA replication ensures that each daughter cell has an exact copy of the genetic material from the parent cell. • DNA polymerases can find and correct errors. Replication is fast and accurate. DNA replication occurs before mitosis begins and before the first division of meiosis. –helicase unwinds the double helix –the two exposed strands of DNA act as a template for DNA replication –DNA polymerase adds the correct complimentary base pairs to the exposed strands Binary Fission • Bacteria reproduce asexually via binary fission • Each daughter cell is an identical copy (or clone) of its parent cell. RepA helicase “melts” the DNA at the replication fork 3. 8.3 DNA Replication There are many origins of replication in eukaryotic chromosomes. Each body cell gets a complete set of identical DNA. 34.2: DNA Replication is Highly Coordinated An Origin for DNA Replication A helicase • Replication involves initiation, elongation, and termination. Process of DNA Replication 3. A single strand of DNA serves as a template for a new strand. DNA serves only as a template. 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